Detection of Spots in 2-D Electrophoresis Gels by Symmetry Features
نویسندگان
چکیده
We have implemented an algorithm for detection and segmentation of protein spots in 2-D gel electrophoresis images using symmetry derivative features computed using low level image processing operations. The implementation was compared with a previously published Watershed segmentation and a commercial software. Our algorithm was found to yield segmentation results that were either better than or comparable to the other solutions while having fewer free parameters and a low computational cost.
منابع مشابه
Proteomic Analysis of Gene Expression in Basal Cell Carcinoma
Background: Basal Cell Carcinoma (BCC) is a type of non-melanoma skin cancer. Alteration in gene expression is the important event that happens in cancer cell. Detection of this event is possible by proteomics techniques. Methods: Normal and tumor tissues were taken from BCC patient. Total proteins were purified by standard methods, and proteins were separated by two-dimensional electrophoresis...
متن کاملCapillary electrophoresis/tandem mass spectrometry for analysis of proteins from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis.
Capillary electrophoresis/time-of-flight mass spectrometry(CE/TOFMS) has been used for analysis of in-gel digests of protein spots excised from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE). An off-line purification and preconcentration procedure with a Zip Tip is used before CE/TOFMS analysis which allows for detection of protein spots with <1 picomol...
متن کاملAnalysis of H-2 and Ia molecules by two-dimensional gel electrophoresis
Mouse lymphocyte H-2 and Ia glycoproteins have been analyzed with a two-dimensional (2-D) acrylamide gel electrophoresis technique, in which proteins are separated first according to their charge in isoelectrofocusing gels and then according to their size in sodium dodecyl sulfate gels. Individual polypeptide chains from radiolabeled cells are resolved as discrete spots on autoradiograms of the...
متن کاملDetection and Quantification of Protein Spots by Pinnacle.
Accurate spot detection and quantification is a challenging task that must be performed effectively in order to properly extract the proteomic information from two-dimensional (2-D) gel electrophoresis images. In Morris et al., Bioinformatics 24:529-536, 2008, we introduced Pinnacle, an automatic, fast, effective noncommercial package for spot detection and quantification for 2-D gel images, an...
متن کاملDe - noising 2 D gel images using Wavelet transform based on genetic algorithm
In proteomics 2-dimensional SDS-polyacrylamide gel electrophoresis (2D-PAGE) is the most widely used method for analyzing protein mixtures qualitatively. There are, however, a lot of noise and measurement biases which needs to be accounted for both in the localization of spots as well as in the quantitative measurement of protein expression. Previous techniques for denoising 2D gels are based o...
متن کامل